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Home The evolution of DNA sequencing and cancer genomics based on next generation sequencing

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Identifier	Thesis 2178
Author	Lahnin, Achraf, 1977-
Title	The evolution of DNA sequencing and cancer genomics based on next generation sequencing
Publisher	Central Connecticut State University
Date	2011; *
Resource Type	Master's Thesis
Notes	The first methods for sequencing DNA were developed in the mid-1970’s (Strausberg et al. 2008). The sequencing efforts were labor-intensive, slow, and costly (Metzker, 2005). As a result, researchers could sequence only a few base pairs per year. By the time the human genome project began in 1990, only a few researchers were able to sequence 100,000 bases (Shendure et al. 2004). Sequencing of large numbers of samples required complex automations in order to obtain high throughput consistent quality (Metzker, 2005). Since then, improved sequencing technologies have allowed the field of genomics to evolve. Exciting new DNA sequencing technologies has lead to the sequencing of many microbial genomes and higher eukaryotes genomes (Metzker, 2005). These technological improvements have not only increased speed, but are also cost effective. Several platforms were discovered and are based on different principles that have differences in sequence read lengths and numbers (Marguerat et al. 2008). New sequencing technologies are rapidly growing and have the potential to speed up research and biomedical research. This thesis describes the history of DNA sequencing technology and describes recent improved state of the art gene sequencing technologies. This thesis will also compare and contrast data obtained from these new sequencing platforms, as well as future prospects of new sequencing technology on cancer research.
Subject	DNA -- Analysis Nucleotide sequence Cancer genes
Department	Department of Biomolecular Sciences
Advisor	Martin-Troy, Kathy
Type	Text
Digital Format	application/pdf
Language	eng
OCLC number	804653130
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