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Home CCSU Theses & Dissertations Identification of four potential A-to-I RNA editing targets in the crustacean Artemia franciscana

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Identification of four potential A-to-I RNA editing targets in the crustacean Artemia franciscana

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Identifier	Thesis 2123
Author	Carlson, Kristina Rebekka, 1980-
Title	Identification of four potential A-to-I RNA editing targets in the crustacean Artemia franciscana
Publisher	Central Connecticut State University
Date	2010
Resource Type	Master's Thesis
Notes	A-to-I RNA editing is an enzymatic reaction commonly found in the nervous system of various organisms. The mechanism behind this post-transcriptional modification is the hydrolytic deamination of Adenosine to Inosine by a family of enzymes known as Adenosine Deaminases that act on RNA (ADAR). ADARs alter the composition of Adenosine to form Inosine in pre-mRNA. The Inosine is read as a Guanosine by the ribosomes during translation. Alterations may occur due to editing during translation that can result in the production of different amino acids from the original genomically-encoded DNA transcript which can affect the proteins produced. Alterations in the protein products have been shown to lead to various diseases in humans; such as neurodegenerative diseases. A-to-I RNA editing sites have been identified in the para sodium channel gene of Drosophilia melanogaster. We hypothesize that there are potential RNA editing targets in the crustacean Artemia franciscana. Our methods include isolating Artemia RNA and DNA for use in an RT-PCR. We designed primers to amplify the sodium channel gene in Artemia and used those primers for a PCR reaction. We were able to sequence the PCR product and found four A/G mixed signals which can be confirmed as editing sites by sequencing genomic DNA. Since editing occurs post-transcriptionally, if the genomic DNA contains an Adenosine at the location of the A/G mixed signal, then it is an actual editing site. If the DNA contains a Guanosine in place of the mixed signal, then the editing site is a polymorphism and not an actual edit.
Subject	RNA editing Adenosine Inosine Sodium channels Artemia
Department	Department of Biomolecular Sciences
Advisor	Hoopengardner, Barry
Type	Text
Digital Format	application/pdf
Language	eng
OCLC number	706471639
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Home CCSU Theses & Dissertations Identification of four potential A-to-I RNA editing targets in the crustacean Artemia franciscana

Identification of four potential A-to-I RNA editing targets in the crustacean Artemia franciscana

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