Log in
|
Help
|
English

English

Engish-Pirate

한국어

Search

Advanced Search

Find results with:

Add another field

Search by date

to

Searching collections:

CCSU Theses and Dissertations

Add or remove collections

Home CCSU Theses & Dissertations Expression of Cyclin D1 in zebrafish embryo

Reference URL

Share

Add tags

Comment

Rate

To link to this object, paste this link in email, IM or document

To embed this object, paste this HTML in website

Expression of Cyclin D1 in zebrafish embryo

View Description

Download

small (250x250 max)

medium (500x500 max)

large ( > 500x500)

Full Resolution

Print

you wish to report:

Your comment:

Your Name:

Submit

Cancel

...

Back to top

Powered by CONTENTdm®

Select the collections to add or remove from your search

A

B

C

D

E

F

G

H

I

J

K

L

M

N

O

P

Q

R

S

T

U

V

W

X

Y

Z

Select All Collections

C

CCSU Student Publications

CCSU Theses and Dissertations

G

GLBTQ Archives

M

Modern Language Oral Histories

O

O'Neill Archives Oral Histories

P

Polish American Pamphlets

T

Treasures from the Special Collections

V

Veterans History Project

OK

Cancel

Identifier	Thesis 2115
Author	Ji, Min, 1969-
Title	Expression of Cyclin D1 in zebrafish embryo
Publisher	Central Connecticut State University
Date	2010
Resource Type	Master's Thesis
Notes	Cyclin D1 is the major regulator of cell cycle --- the division of cells to copy themselves. Cyclin D1 is the product of ccnd1 gene and plays an important role in regulation of cell cycle transition from G1 phase to S phase. The cell cycles of zebrafish embryonic cells in the developmental early stage are very short and synchronous. DNA replication (S phase) and mitosis (M phase) can be observed, but there is not detectable G1 or G2 phase of the cell cycle in zebrafish early developmental stage. Subsequently, cell cycles gradually lengthen and cells gradually lose synchrony during the embryonic development. It will be very important to know when and where the ccnd1 starts to express in zebrafish embryo during the embryogenesis. We have implemented in situ hybridization to localize the ccnd1 gene’s transcript during zebrafish embryogenesis. We have found that ccnd1 gene is expressed in the surface of germ ring during gastrulation period of zebrafish embryo, which is as early as the stage at 5.7 hpf (hours post fertilization). MyoD is an important gene regulating muscle formation during embryogenesis. MyoD and cyclin D1 had been reported to antagonize each other. To better understand how cyclin D1 and MyoD regulate each other, we have also localized MyoD gene transcript in zebrafish embryo by in situ hybridization. We have found that MyoD expressed in the tail of embryo dorsal side at the shield stage (6 hpf) following the stage of ccnd1 expression. MyoD transcript-localized cells formed a unique pattern. The patches of cells with MyoD transcript elongated and formed a pair of narrow longitudinal rows, which lay on either side of the prospective notochord. The expressing patterns and locations of two genes are different. Some locations of two genes’ transcripts seem to overlap. Further experiments using fluorescent in situ hybridization (FISH) need to be done to detect both ccnd1 and MyoD genes’ transcripts in the same cells in the embryo to check if both genes can be co-localized in the same cells in different stage of embryogenesis.
Subject	Cyclins Zebra danio Cells -- Growth -- Regulation
Department	Department of Biomolecular Sciences
Advisor	Martin-Troy, Kathy
Type	Text
Digital Format	application/pdf
Language	eng
OCLC number	703420784
Rating

Find results with:

Searching collections:

Home CCSU Theses & Dissertations Expression of Cyclin D1 in zebrafish embryo

Expression of Cyclin D1 in zebrafish embryo

Description

Tags

Comments