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A fluorescence in situ hybridization (FISH) analysis of human lung cancer / Denise E. Anamani
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A fluorescence in situ hybridization (FISH) analysis of human lung cancer / Denise E. Anamani
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Description
Identifier
Thesis
1749
Author
Anamani, Denise E., 1963-
Title
A
fluorescence
in
situ
hybridization
(FISH)
analysis
of
human
lung
cancer
/
Denise
E
.
Anamani
Publisher
Central Connecticut State University
Date
2004
Resource Type
Master's Thesis
Notes
This
study
examines
the
relationship
ovserved
between
the
chromosomal
abnormalities
detected
by
FISH
and
available
clinical
data
on
cancer
type
on
forty
lung
cancer
specimens
. An
attempt
to
derive
correlation
between
subtype
of
lung
cancer
and
type
or
severity
of
genetic
abnormality
observed
with
RSH
was
undertaken
. Any
such
relationship
found
will
provide
further
evidence
of the
usefulness
of
FISH
in
caner
diagnosis
and
management
.
Fluorescence
in
situ
hybridization
was
performed
on
archival
(paraffinembedded)
human
lung
cancer
tissue
samples
from the
Department
of
Pathology
at
Hartford
Hospital
in
Hartford
,
Connecticut
. The
obtaining
of
specimens
and
clinical
data
was
done
so
that the
researcher
was
blinded
to
all
patient
information
throughout
the
entire
study
. The
Vysis
LAVysionTM
probe
set
was
used
for
FISH
analysis
.
Hybridized
slides
were
viewed
with a
fluorescent
microscope
and the
hybridization
signals
for the
four
LAVysionTM
probes
were
recorded
on
count
sheets
. For
each
slide
,
forty
nuclei
were
enumerated
.
One
hundred
per
cent
of the
lung
cancer
cases
examined
were
positive
for
genetic
abnormalities
as
determined
by
LAVysionTM
. The
samples
comprised
four
major
subtypes
of
lung
cancer
.
Adenosquamous
carcinoma
cases
showed
the
most
dramatic
genetic
anomalies
.
Overall
the
most
common
genetic
abnormality
seen
was
gain
in
gene
number
at the
c-myc
locus
.
Subject
Fluorescence in situ hybridization
Lungs -- Cancer
Department
Department of Biological Sciences
Advisor
Martin-Troy, Kathy
Type
Text
Digital Format
application/pdf
Language
eng
OCLC number
57671022
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