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Home CCSU Theses & Dissertations Development of an enzyme linked immunosorbant assay for quantitative detection of murine...

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Development of an enzyme linked immunosorbant assay for quantitative detection of murine anti-human antibodies / Paul Barboni

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Identifier	Thesis 1750
Author	Barboni, Paul, 1974-
Title	Development of an enzyme linked immunosorbant assay for quantitative detection of murine anti-human antibodies / Paul Barboni
Publisher	Central Connecticut State University
Date	2004
Resource Type	Master's Thesis
Notes	During the development of potential protein or antibody therapeutics in a pharmaceutical environment, the use of animal models as test subjects is a necessary requirement. The dosing of mice, rats, and primates is essential to drug development because this can provide pharmacokinetic and toxicological information in mammalian applications prior to the human dosing phase of this development (Digit et al. 2003, Frantz et al. 1994, Zhong et al. 2000). Effective doses can be estimated from these trials, as can deleterious and dangerous doses (Foster 1993, Montgomery 2001, Parchment 1993, Pessina 2002). The side effects and benefits of the therapeutic become evident during this animal model phase (Hodge 2003, Lappin 2003, Mirsalis 2003, Shojaee 2003). To test the pharmacokinetics of the drug in these animals there are several key areas of information that are of interest to researchers regarding the behavior of the protein or antibody therapeutic after dosing. Questions of drug target and metabolism are very important in therapeutic development, as are the subjects' reactions to the dose. Any immune response to the dose is not only important to understanding the treatment pathway, but can also help explain the results of animal trials (Kim 2001, Phillips 1999, Weijzen 2001). Antibodies made by the host in response to the protein treatment would be of great importance in interpreting the results of animal trials, and a method of measuring such antibodies would be a valuable tool in this interpretation. An enzyme-linked immunosorbant assay (ELISA) was designed to quantitatively measure the amounts of antibodies present in the sera of mice that have been dosed with a human antibody therapeutic. This assay can subsequently be employed in applicable studies, and the methodology used in its development can also be applied to the creation of other essays.
Subject	Antibody-directed enzyme prodrug therapy
Department	Department of Biomolecular Sciences
Advisor	Martin-Troy, Kathy
Type	Text
Digital Format	application/pdf
Language	eng
OCLC number	57705183
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