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Vesicular packaging of serotonin by astrocytes

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Identifier	Thesis 1908
Author	Ferrazzoli, Alexander E.
Title	Vesicular packaging of serotonin by astrocytes
Publisher	Central Connecticut State University
Date	2007
Resource Type	Master's Thesis
Notes	Abnormalities in the synaptic concentration of serotonin are often linked to clinical and psychiatric disorders. This concentration of serotonin is regulated, by serotonergic neurons, through a cycle of serotonin synthesis and vesicular packaging, via the vesicular monoamine transporter type 2 (VMAT-2), calcium-mediated exocytosis, reuptake via the serotonin reuptake transporter (SERT), degradation via the mitochondrial monoamine oxidase system (MAO), and repackaging in vesicles. This cycle is known as the serotonergic system. Astrocytes are known to contribute to the serotonergic system through the uptake of serotonin and subsequent degradation. This uptake is known to be Na+-dependent and fluoxetine sensitive. This project began by investigating the uptake of serotonin in primary culture astrocytes. Astrocytes are dissociated from 1-3 day old rat pups, cultured for approximately 10 days, and then exposed to 0.3 μM serotonin for 5 hours. Immunofluorescent microscopy results have uncovered localization of serotonin in astrocytes after uptake. This localization may be due to packaging in vesicles via VMAT-2. Glial fibrillary acidic (GFAP)-positive, an astrocyte specific marker, astrocytes have been probed with antibodies for serotonin and VMAT-2, and co-localization of both has been found. The presence of VMAT-2 through amplification of the VMAT-2 transcript by RT-PCR has been verified. These results suggest that astrocytes may be capable of packaging serotonin after reuptake and, therefore, may play a larger role in the serotonergic system than was previously believed. These studies may help uncover novel pharmaceutical targets for the development of treatments for serotonin-related clinical and psychiatric disorders.
Subject	Serotonin Astrocytes
Department	Department of Biomolecular Sciences
Advisor	Watson, Cheryl L
Type	Text
Digital Format	application/pdf
Language	eng
OCLC number	713734001
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