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Home Transcriptome analysis of the synganglion of the tick, Ixodes scapularis

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Transcriptome analysis of the synganglion of the tick, Ixodes scapularis

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Identifier	Thesis 1973
Author	Huang, Jianping
Title	Transcriptome analysis of the synganglion of the tick, Ixodes scapularis
Publisher	Central Connecticut State University
Date	2008
Resource Type	Master's Thesis
Notes	The tick has ability to transmit a greater variety of infectious agents to humans and other animal species than any other blood-feeding arthropod. Feeding over a period of days induces specific host hemostatic, inflammatory, and immune responses. The nervous and neuroendocrine systems of the tick are central components in all aspects of tick feeding, including responses to changes in the host environment of the bite site. Control of ticks and tick-borne diseases is dependent upon application of acaricides to livestock and use of repellents for personal protection of humans. Tick resistance to acaricides is a significant problem and there exists a need to identify new targets for chemical control of ticks. A genomic approach to characterize the synganglia of unfed and feeding ticks provides a powerful tool to understanding the functions of this important organ and the identification of novel targets for development of acaricides. The entire central nervous system in ticks is a highly condensed, nerve mass consisting of cerebral ganglia, the ventral nerve cord and the portion of the tick ganglia collectively known as the periesophageal synganglion. he synganglion is also a neurosecretory center in which neurotransmitter imports pass through a complex system of neurosecretory tracts to reach target organs. Thus, tick synganglion and axonal pathways in the peripheral nerves serve as the neuroendocrine system of this blood feeding arthropod. The present work uses cDNA sequence analyses to identify Ixodes scapularis synganglion transcripts. Ixodes scapularis is a vector of the causative agents of Lyme borreliosis, Borrelia burgdorferi, human granulocytic anaplasmosis, Anaplasma phagocytophilum, babesiosis, Babesia microti, and the North American subtype of tick-borne encephalitis virus. Automated single-pass DNA sequencing was performed on 3360 randomly selected complementary DNA (cDNA) clones from the synganglia of fed (4-5 days) and unfed adult female I. scapularis. Analysis of the expressed sequence tags (ESTs) resulted in identification of 2006 singletons and 248 clusters with more than one quality read. Attempts were made to assign putative functions to tick synganglion genes based on putative amino acid identity to records published in public protein databases. Approximately 58 % (1311) of the sequences showed limited or no homology to previously identified gene products. A number of sequences were identified which shared significant sequence similarity to mammalian genes normally associated with neurotransmitter transport, neurotoxin, neuropeptides, and neurosecretion related protein hormones. Several coding sequences possessed various degrees of homology to previously reported proteins from ticks. Selected genes identified as differentially expressed were sequenced and identified by comparison to the I. scapularis genome database. Identification of novel and/or differentially expressed synganglion genes may result in identification of target molecules for novel acaricide development.
Subject	Ixodes scapularis
Department	Department of Biomolecular Sciences
Advisor	Martin-Troy, Kathy
Type	Text
Digital Format	application/pdf
Language	eng
OCLC number	713734059
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Home Transcriptome analysis of the synganglion of the tick, Ixodes scapularis

Transcriptome analysis of the synganglion of the tick, Ixodes scapularis

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