Skip to main content
Home
Browse All
Log in
|
Favorites
|
Help
|
English
English
Engish-Pirate
한국어
Search
Advanced Search
Find results with:
error div
Add another field
Search by date
Search by date:
from
after
before
on
from:
to
to:
Searching collections:
CCSU Theses and Dissertations
Add or remove collections
Home
CCSU Theses & Dissertations
Refining the Location of the frizzy (fr) Mutation on Mus musculus Chromosome 7
Reference URL
Share
Add tags
Comment
Rate
Save to favorites
Remove from favorites
To link to this object, paste this link in email, IM or document
To embed this object, paste this HTML in website
Refining the Location of the frizzy (fr) Mutation on Mus musculus Chromosome 7
View Description
Download
small (250x250 max)
medium (500x500 max)
Large
Extra Large
large ( > 500x500)
Full Resolution
Print
1089.pdf
Description
Identifier
Thesis
1881
Author
Thompson, David S.
Title
Refining
the
Location
of the
frizzy
(fr)
Mutation
on
Mus
musculus
Chromosome
7
Publisher
Central Connecticut State University
Date of Publication
2006
Resource Type
Master's Thesis
Abstract
The
frizzy
(fr)
mutation
in
Mus
musculus
affects
hair
structure
and
formation
.
Early
studies
demonstrated
that the
gene
responsible
for
frizzy
assorts
to
mouse
Chromosome
7
along
with
pink-eyed
dilution
(p)
,
chinchilla
(cch)
, and
shaker-1
(sh-1)
. These
last
three
phenotypic
traits
have been
mapped
with
respect
to
modern
molecular
markers
and
assigned
to
known
genes
, but
fr
has not.
Given
that
autosomal
recessive
genes
affecting
coat
color
and
development
frequently
evince
pleiotropic
effects
,
it
could
be
valuable
to
understand
in
detail
what the
molecular
basis
of the
frizzy
mutation
is
, what
role(s)
the
wild
type
gene
plays
in
normal
mouse
development
, and how the
frizzy
mutation
disrupts
normal
development
.
Prior
to
undertaking
such
an
analysis
,
however
,
we
need
to
identify
the
gene
that
harbors
the
fr
defect
.
Recent
complementation
testing
demonstrated
both
that
fr
is
not
allelic
with
fibroblast
growth
factor
receptor
2
(Fgfr2
–
a
critical
protein
contributing
to the
formation
of
multiple
tissue
types)
, and that
fr
lies
centromeric
of
Fgfr2
. With
Fgfr2
established
as the
telomeric
flank
for the
fr
region
, and
sh-1
the
only
known
centromeric
flank
,
we
proposed
to
develop
a
fine-structure
map
of the
interval
between
sh-1
and
Fgfr2
;
hoping
thus
to
isolate
fr
to an
interval
containing
no
more
than
about
20
genes
.
Using
a
backcross
of
standard
inbred
strains
we
generated
a
panel
of
93
mice
that
segregated
for
frizzy
.
FS/EiJ
(FS)
provided
the
strain
homozygous
for
fr
.
C57BL/6J
(B6)
provided
the
wild
type
strain
.
After
extracting
DNA
from
distal
tail
tips
,
we
probed
each
sample
for
PCR
scorable
molecular
markers
to
determine
for
each
marker
whether
it
traveled
with or had
recombined
with
fr
.
Using
classical
haplotype
analysis
we
have
identified
progressively
closer
flanks
for the
interval
containing
fr
.
Our
starting
32
megabase-pair
(Mbp)
interval
contained
over
530
genes
.
While
we
were not
able
to
confine
fr
to a
20-gene
interval
,
we
have
significantly
reduced
the
search
interval
to the
6
Mbp
between
D7MIT41
on the
centromeric
flank
and
D7MIT165
on the
telomeric
flank
. This
interval
,
contains
nearly
130
known
genes
;
too
large
a
number
for
candidate
screening
by
tissue
expression
association
and
sequencing
. To
reach
the
goal
of
localizing
fr
to a
region
that
includes
only
a
small
number
of
candidate
genes
,
further
mapping
analysis
is
recommended
. If
more
B6
vs
.
FS
dimorphic
markers
can
be
found
, the
93-member
panel
described
here
might
be
exploited
to
further
restrict
the
critical
interval
, but the
microsatellite
markers
currently
available
between
D7MIT41
and
D7MIT165
have not
proven
productive
with
respect
to the
amplimer
products
we
resolved
using
3.5%
NuSieve
agarose
gels
.
Additional
sequence-level
B6
vs
.
FS
dimorphisms
in the
D7MIT41
to
D7MIT165
interval
might
be
found
either
by
repeating
the
current
procedures
using
polyacrylamide
gels
to
improve
our
ability
to
discern
amplimer
length
differences
less
than
10
base
pairs
, or by
seeking
other
types
of
markers
. In
either
case
,
given
the
high
gene
density
found
in the
critical
region
(on
average
we
expect
to
find
about
60
genes
in a
6
Mbp
interval)
,
more
recombinant
mice
will
likely
be
needed
to
achieve
the
desired
map
resolution
. To
increase
both
the
number
of
recombinants
and the
ease
of
discovering
marker
dimorphisms
,
it
will
probably
be
most
productive
to
conduct
a
new
backcross
using
FS
and
whichever
standard
strain
(i.e.
,
BALB
,
A/J
,
129
, or
DBA/2)
harbors
the
most
sequence
level
differences
compared
to
FS
.
Subject
Mice -- Genetics
Department
Department of Biomolecular Sciences
Advisor
King, Thomas R.
Type
Text
Digital Format
application/pdf
Language
eng
OCLC number
713734335
Rating
Tags
Add tags
for Refining the Location of the frizzy (fr) Mutation on Mus musculus Chromosome 7
View as list
|
View as tag cloud
|
report abuse
Comments
Post a Comment
for
Refining the Location of the frizzy (fr) Mutation on Mus musculus Chromosome 7
Your rating was saved.
you wish to report:
Your comment:
Your Name:
...
Back to top
Select the collections to add or remove from your search
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Select All Collections
C
CCSU Student Publications
CCSU Theses and Dissertations
G
GLBTQ Archives
M
Modern Language Oral Histories
O
O'Neill Archives Oral Histories
P
Polish American Pamphlets
Polish Posters
T
Treasures from the Special Collections
V
Veterans History Project
500
You have selected:
1
OK
Cancel