Identifier	Thesis 1998
Author	Bannock, Jason Michael
Title	RNA Editing of Voltage-Gated Ion Channels Over Time
Publisher	Central Connecticut State University
Date of Publication	2008
Resource Type	Master's Thesis
Abstract	RNA editing is a mechanism which is used to increase the number of possible protein products from a specific gene. The catalytic enzymes for RNA editing are known to be present in a wide variety of organisms, including many different insects. It has been shown that the frequency of RNA editing fluctuates between the following stages of development, egg/larva, larva, pupae, and adult. Our goal was to use a known editing site and measure the frequency of editing as the adult fly ages. A-to-I RNA editing is an enzymatic process in which Adenosine is converted to Inosine post-transcriptionally by the ADAR family of enzymes. Analysis has been done in the Slowpoke potassium ion channel to observe the frequency at which RNA editing of this transcript occurs. In order to detect RNA editing using sequencing, it is necessary to convert RNA transcripts to DNA products for sequencing. RT-PCR is a technique that utilizes the enzyme reverse transcriptase to convert an RNA molecule into a complementary DNA (cDNA) molecule that can then be used as a template for a PCR reaction. The PCR product may then be sequenced. RNA editing is detectable as mixed signals in sequencing chromatograms, with controls to distinguish editing from DNA polymorphism. Through this research it was determined that the frequency at which the RNA is edited in the Slowpoke potassium ion channel shows an increase from young adult flies (1-3 days after hatching) to old adults (35-36 days after hatching). Ultimately the goal is to obtain RNA editing sites unique to single insect species that could then be used for the possible development of species-specific pesticides.
Subject	RNA editing
Department	Department of Biomolecular Sciences
Advisor	Hoopengardner, Barry
Type	Text
Digital Format	application/pdf
Language	eng
OCLC number	713734076
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RNA Editing of Voltage-Gated Ion Channels Over Time

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