The intent of this research project was to purify the Shigella flexneri virulence plasmid without the use of ultra-centrifugation. The ultra-centrifugation is the most commonly used technique; however the PFGE has proven to isolate the plasmid...
The prophage of bacteriophage P1 is a unit copy plasmid, stable in Escherichia coli during cell division due to its partition system. The active P1 partition system consists of two genes, parA and parB, and a cis-site, parS. ParS contains all of...
