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Adrenal gland influence on the host TNF-a response to lipopolysaccharide (LPS) and monophosphoryl...

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Adrenal gland influence on the host TNF-a response to lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA) : evaluation of TNF-a mRNA transcription

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Identifier	Thesis 1405
Author	Amador-Medina, Ivette.
Title	Adrenal gland influence on the host TNF-a response to lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA) : evaluation of TNF-a mRNA transcription
Publisher	Central Connecticut State University
Date of Publication	1995
Resource Type	Master's Thesis
Abstract	Lipopolysaccharide (LPS) and the non-toxic derivative of lipid A, Monophosphoryl Lipid A (MPLA) were utilized to evaluate the influence of the adrenal gland on the intensity of the murine host response to endotoxin. Adrenal sufficient (intact) and surgically adrenalectomized Swiss Webster random bred female mice were challenged with endotoxin. Adrenalectomized mice were injected intraperitoneally (IP) with 2 mg/kg or 10 mg/kg MPLA or 2 mg/kg LPS. Intact mice received 2 mg/kg LPS or 20 mg/kg MPLA. Total tissue RNA was isolated at 45, 90 and 180 minutes following endotoxin challenge and analyzed for TNFa mRNA by solution hybridization. Autoradiogram intensity was image analyzed and the calculated response values included normalization with B-actin. Both LPS and MPLA induced expression of TNF a as assessed by increased steady state mRNA levels in spleen and lung tissues. TNFa mRNA transcript ratios increased in lung and spleen tissues following LPS challenge, but not MPLA challenge in adrenal sufficient mice. LPS challenged adrenalectomized mice showed no significant increase in TNFa mRNA, while MPLA challenged mice showed an increase in TNFa mRNA. Differential expression of TNFa mRNA in response to MPLA and LPS endotoxin challenge was observed in lung and spleen tissues of intact as compared to adrenalectomized mice. These results suggest that adrenal corticosteroids possibly influence bioactive TNFa production in vivo at the transcriptional and translational level following endotoxin challenge.
Notes	"Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Arts in Biology."; Thesis advisor: Thomas R. King.; M.A.,Central Connecticut State University,1995; Includes bibliographical references (leaves 62-68).
Subject	Endotoxins. Tumor necrosis factor. Adrenocortical hormones.
Department	Department of Biological Sciences
Advisor	King, Thomas R.
Type	Text
Software	System requirements: PC and World Wide Web browser.
Language	eng
OCLC number	36222698
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Adrenal gland influence on the host TNF-a response to lipopolysaccharide (LPS) and monophosphoryl lipid A (MPLA) : evaluation of TNF-a mRNA transcription

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