Skip to main content
Home
Browse All
Log in
|
Favorites
|
Help
|
English
English
Engish-Pirate
한국어
Search
Advanced Search
Find results with:
error div
Add another field
Search by date
Search by date:
from
after
before
on
from:
to
to:
Searching collections:
CCSU Theses and Dissertations
Add or remove collections
Home
CCSU Theses & Dissertations
Discovery of RNA editing in the grin1b transcipt of zebrafish (Danio rerio): a predictive and...
Reference URL
Share
Add tags
Comment
Rate
Save to favorites
Remove from favorites
To link to this object, paste this link in email, IM or document
To embed this object, paste this HTML in website
Discovery of RNA editing in the grin1b transcipt of zebrafish (Danio rerio): a predictive and experimental approach
View Description
Download
small (250x250 max)
medium (500x500 max)
Large
Extra Large
large ( > 500x500)
Full Resolution
Print
1648.pdf
Description
Identifier
Thesis
2143
Author
Pozo, Pedro Nicolas,1984-
Title
Discovery
of
RNA
editing
in the
grin1b
transcipt
of
zebrafish
(Danio
rerio)
: a
predictive
and
experimental
approach
Publisher
Central Connecticut State University;
Date of Publication
2011
Resource Type
Master's Thesis
Abstract
Discovering
RNA
editing
sites
in
model
organisms
provides
an
insight
into their
adaptations
in
addition
to
finding
potential
sites
for
gene
expression
regulation
. In this
study
,
we
focused
on the
grin1b
gene
of
Zebrafish
due
to its
important
function
in the
nervous
tissue
as a
glutamate
receptor
ion
channel
.
We
used
NCBI
computer
programs
to
locate
5
possible
RNA-editing
events
within
the
grin1b
transcript
and
designed
and
ordered
primers
targeted
at these
possible
editing
sites
.
One
primer
pair
,
F3R3
,
gave
us a
robust
band
at the
size
predicted
which
upon
sequencing
identified
E5
as a
potential
editing
site
; this was
verified
by
DNA
controls
.
Surprisingly
,
sequence
analysis
also
revealed
a
new
editing
site
,
E6
,
which
was not
observed
in the
predictive
computer
models
. By
comparing
these
cDNA
sequences
to the
genomic
sequence
of
grin1b
as a
control
,
we
show
for the
first
time
the
discovery
of
two
novel
RNA
editing
events
in the
grin1b
transcript
of
Zebrafish
.
Studying
the
frequency
levels
of these
events
with the
help
of
restriction
enzymes
and
densitometry
analyses
enabled
us to
further
characterize
this
novel
finding
.
Editing
at
site
E5
created
a
restriction
enzyme
site
for the
endonuclease
MluI
,
whereas
editing
at
site
E6
created
a
restriction
enzyme
site
for the
endonuclease
BstNI
.
Upon
completion
of
restriction
digests
using
these
enzymes
and by
using
densitometry
analyses
we
found
the
frequency
of
editing
at
site
E5
to be
26.98%
and
site
E6
to be
21.36%
.
Collectively
, this
study
shows
for the
first
time
the
discovery
of
RNA
editing
in the
grin1b
gene
transcript
of
Zebrafish
. This
exciting
finding
represents
the
first
time
RNA
editing
is
observed
in this
glutamate
receptor
ion
channel
and
provides
further
insight
into this
molecular
process
.
Moreover
, by
characterizing
this
finding
in
terms
of
frequency
and
location
we
hope
to
advance
our
current
knowledge
of
RNA
editing
with the
ultimate
goal
of
elucidating
this
intriguing
molecular
process
.
Subject
RNA editing
Zebra danio
Department
Department of Biomolecular Sciences
Advisor
Hoopengardner, Barry
Type
Text;
Digital Format
application/pdf
Language
eng
OCLC number
804652989
Rating
Tags
Add tags
for Discovery of RNA editing in the grin1b transcipt of zebrafish (Danio rerio): a predictive and experimental approach
View as list
|
View as tag cloud
|
report abuse
Comments
Post a Comment
for
Discovery of RNA editing in the grin1b transcipt of zebrafish (Danio rerio): a predictive and experimental approach
Your rating was saved.
you wish to report:
Your comment:
Your Name:
...
Back to top
Select the collections to add or remove from your search
A
B
C
D
E
F
G
H
I
J
K
L
M
N
O
P
Q
R
S
T
U
V
W
X
Y
Z
Select All Collections
C
CCSU Student Publications
CCSU Theses and Dissertations
G
GLBTQ Archives
M
Modern Language Oral Histories
O
O'Neill Archives Oral Histories
P
Polish American Pamphlets
Polish Posters
T
Treasures from the Special Collections
V
Veterans History Project
500
You have selected:
1
OK
Cancel